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DNMT3A Polyclonal antibody, PBS Only

Name: DNMT3A Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 20954-1-PBS

DNMT3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Review Product

Cat no : 20954-1-PBS

Synonyms

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 20954-1-AP (same clone as 20954-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)
WB result of DNMT3A antibody (20954-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNMT3A transfected HepG2 cells. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
WB analysis using 20954-1-AP (same clone as 20954-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)
IP result of anti-DNMT3A (IP:20954-1-AP, 4ug; Detection:20954-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

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Cat No. 20954-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20954-1-PBS targets DNMT3A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNMT3A fusion protein Ag15111 相同性解析による交差性が予測される生物種
Full Name	DNA (cytosine-5-)-methyltransferase 3 alpha
Calculated molecular weight	912 aa, 102 kDa
Observed molecular weight	120-130 kDa
GenBank accession number	BC043617
Gene symbol	DNMT3A
Gene ID (NCBI)	1788
RRID	AB_10733339
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNMT3A Polyclonal antibody, PBS Only

DNMT3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Validation Data Gallery

WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)

WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of human stomach cancer using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of human stomach cancer using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)

IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)

IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)

IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

Tested Applications

Recommended dilution

Product Information

Background Information