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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNMT3A Polyclonal antibody (20954-1-AP)

    WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DNMT3A Polyclonal antibody (20954-1-AP)

    WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)

    WB result of DNMT3A antibody (20954-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNMT3A transfected HepG2 cells. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DNMT3A Polyclonal antibody (20954-1-AP)

    WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DNMT3A Polyclonal antibody (20954-1-AP)

    IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    IP result of anti-DNMT3A (IP:20954-1-AP, 4ug; Detection:20954-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using DNMT3A Polyclonal antibody (20954-1-AP)

    IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using DNMT3A Polyclonal antibody (20954-1-AP)

    IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNMT3A Polyclonal antibody (20954-1-AP)

    IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DNMT3A Polyclonal antibody, PBS Only

DNMT3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20954-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

M.HsaIIIA, EC:2.1.1.37, EC:2.1.1.-, DNA MTase HsaIIIA, DNA (cytosine-5)-methyltransferase 3A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20954-1-PBS targets DNMT3A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT3A fusion protein Ag15111 相同性解析による交差性が予測される生物種
Full Name DNA (cytosine-5-)-methyltransferase 3 alpha
Calculated molecular weight 912 aa, 102 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC043617
Gene Symbol DNMT3A
Gene ID (NCBI) 1788
RRIDAB_10733339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6K1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.