Featured Product
KD/KO Validated

DNMT3B Polyclonal antibody

Name: DNMT3B Polyclonal antibody
Brand: Proteintech
SKU: 26971-1-AP

DNMT3B Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26971-1-AP

Synonyms

ICF, EC:2.1.1.37, DNMT3, DNMT, DNA (cytosine-5)-methyltransferase 3B

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 26971-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 26971-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 26971-1-AP
IP result of anti-DNMT3B (IP:26971-1-AP, 4ug; Detection:26971-1-AP 1:5000) with K-562 cells lysate 1800 ug.
IHC staining of mouse brain using 26971-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26971-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 26971-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 26971-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 26971-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 26971-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

-/ -

Cat No. 26971-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, K-562 cells, MCF-7 cells, Jurkat cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse brain tissue, human breast cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 20 publications below
IHC	See 5 publications below
IF	See 5 publications below
CoIP	See 2 publications below
ChIP	See 1 publications below

Product Information

26971-1-AP targets DNMT3B in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNMT3B fusion protein Ag25117 相同性解析による交差性が予測される生物種
Full Name	DNA (cytosine-5-)-methyltransferase 3 beta
Calculated molecular weight	95 kDa
Observed molecular weight	85-96 kDa
GenBank accession number	NM_001207055
Gene symbol	DNMT3B
Gene ID (NCBI)	1789
RRID	AB_2880705
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.

Protocols

Product Specific Protocols
WB protocol for DNMT3B antibody 26971-1-AP	Download protocol
IHC protocol for DNMT3B antibody 26971-1-AP	Download protocol
IP protocol for DNMT3B antibody 26971-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	IF,WB	Protein Cell The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting Authors - Yuhan Chen View Article
human	WB	Front Cell Dev Biol miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis Authors - Dong Pan View Article
rat	IHC	Andrology Methylation of eNOS in the rat penile corpus cavernosum under different pathological states and its relationship with erectile function Authors - Na Wang View Article
human	WB	Brain Sci Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes. Authors - Kalaiselvi Sivalingam View Article
human	WB	Biol Res DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice. Authors - Ying Zhu View Article
human	WB	Integr Cancer Ther Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53. Authors - Liang Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNMT3B Polyclonal antibody

DNMT3B Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 26971-1-AP

WB analysis using 26971-1-AP

IP experiment of K-562 using 26971-1-AP

IHC staining of mouse brain using 26971-1-AP

IHC staining of mouse brain using 26971-1-AP

IHC staining of human breast cancer using 26971-1-AP

IHC staining of human breast cancer using 26971-1-AP

IHC staining of mouse testis using 26971-1-AP

IHC staining of mouse testis using 26971-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting

miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis

Methylation of eNOS in the rat penile corpus cavernosum under different pathological states and its relationship with erectile function

Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes.

DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.

Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.