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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNMT3L Polyclonal antibody

DNMT3L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14939-1-AP

Synonyms

DNMT3L

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of PC-3 cells using DNMT3L Polyclonal antibody (14939-1-AP)

    WB analysis of PC-3 using 14939-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14939-1-AP (DNMT3L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DNMT3L Polyclonal antibody (14939-1-AP)

    WB analysis of mouse testis using 14939-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14939-1-AP (DNMT3L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DNMT3L Polyclonal antibody (14939-1-AP)

    WB analysis of HEK-293 using 14939-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14939-1-AP (DNMT3L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14939-1-AP targets DNMT3L in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT3L fusion protein Ag6822 相同性解析による交差性が予測される生物種
Full Name DNA (cytosine-5-)-methyltransferase 3-like
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa and 70 kDa
GenBank accession numberBC002560
Gene symbol DNMT3L
Gene ID (NCBI) 29947
RRIDAB_2094281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNMT3L antibody 14939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
View Article
humanWB

J Reprod Immunol

Decreased expression and DNA hypermethylation of syncytin-1 in human villus tissues with unexplained recurrent spontaneous abortion

Authors - Yang Fu
View Article