Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, 4T1 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67296-1-Ig targets DOCK1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | DOCK1 fusion protein Ag18069 相同性解析による交差性が予測される生物種 |
| Full Name | dedicator of cytokinesis 1 |
| Calculated molecular weight | 1865 aa, 215 kDa |
| Observed molecular weight | 215 kDa |
| GenBank accession number | BC146857 |
| Gene Symbol | DOCK1 |
| Gene ID (NCBI) | 1793 |
| RRID | AB_2882560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14185 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DOCK1, also named as DOCK180, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. DOCK1 functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DOCK1 antibody 67296-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |