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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DOCK180 Polyclonal antibody, PBS Only

DOCK180 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23421-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of HEK-293 using 23421-1-AP (same clone as 23421-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of A431 using 23421-1-AP (same clone as 23421-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of MCF-7 using 23421-1-AP (same clone as 23421-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of LNCaP using 23421-1-AP (same clone as 23421-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DOCK180 Polyclonal antibody (23421-1-AP)

    IP experiment of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

    IP result of anti-DOCK180 (IP:23421-1-AP, 4ug; Detection:23421-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK180 Polyclonal antibody (23421-1-AP)

    IHC staining of human colon using 23421-1-AP (same clone as 23421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK180 Polyclonal antibody (23421-1-AP)

    IHC staining of human colon using 23421-1-AP (same clone as 23421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK180 Polyclonal antibody (23421-1-AP)

    IF Staining of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23421-1-AP (DOCK180 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23421-1-PBS targets DOCK180 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOCK180 fusion protein Ag18069 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 1
Calculated molecular weight 1865 aa, 215 kDa
Observed molecular weight 215 kDa
GenBank accession numberBC146857
Gene symbol DOCK1
Gene ID (NCBI) 1793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.