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  • Featured Product
  • KD/KO Validated

DOCK2 Monoclonal antibody

DOCK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D7A8

Cat no : 66969-1-Ig

Synonyms

dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    WB analysis of Jurkat using 66969-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    WB analysis of Ramos using 66969-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    WB analysis of HeLa using 66969-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DOCK2 Monoclonal antibody (66969-1-Ig)

    IHC staining of human liver cancer using 66969-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DOCK2 Monoclonal antibody (66969-1-Ig)

    IHC staining of human liver cancer using 66969-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK2 Monoclonal antibody (66969-1-Ig)

    IF Staining of HeLa using 66969-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK2 antibody (66969-1-Ig, Clone: 1D7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Ramos cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66969-1-Ig targets DOCK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DOCK2 fusion protein Ag28582 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 2
Calculated molecular weight 212 kDa, 38 kDa
Observed molecular weight 200-210 kDa
GenBank accession numberBC016996
Gene symbol DOCK2
Gene ID (NCBI) 1794
RRIDAB_2882291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.

Protocols

Product Specific Protocols
WB protocol for DOCK2 antibody 66969-1-IgDownload protocol
IHC protocol for DOCK2 antibody 66969-1-IgDownload protocol
IF protocol for DOCK2 antibody 66969-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.

Authors - Mengqi Chu
  • KD Validated
View Article