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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DOCK2-Specific Polyclonal antibody

DOCK2-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20245-1-AP

Synonyms

dedicator of cytokinesis 2, DOCK2, DOCK2-Specific, KIAA0209

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK2-Specific Polyclonal antibody (20245-1-AP)

    WB analysis using 20245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20245-1-AP (DOCK2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using DOCK2-Specific Polyclonal antibody (20245-1-AP)

    IF Staining of Jurkat using 20245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DOCK2-Specific antibody (20245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, Ramos cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20245-1-AP targets DOCK2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 2
Calculated molecular weight 212 kDa
Observed molecular weight212 kDa
GenBank accession numberNM_004946
Gene symbol DOCK2
Gene ID (NCBI) 1794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.

Protocols

Product Specific Protocols
WB protocol for DOCK2-Specific antibody 20245-1-APDownload protocol
IF protocol for DOCK2-Specific antibody 20245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols