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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

DOCK3; MOCA Polyclonal antibody

DOCK3; MOCA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20683-1-AP

Synonyms

dedicator of cytokinesis 3, DOCK3, DOCK3; MOCA, KIAA0299, MOCA, Modifier of cell adhesion, PBP, Presenilin binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK3; MOCA Polyclonal antibody (20683-1-AP)

    WB analysis using 20683-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DOCK3; MOCA Polyclonal antibody (20683-1-AP)

    WB analysis of human brain using 20683-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DOCK3; MOCA Polyclonal antibody (20683-1-AP)

    WB analysis of human brain using 20683-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using DOCK3; MOCA Polyclonal antibody (20683-1-AP)

    WB analysis of SH-SY5Y using 20683-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DOCK3; MOCA Polyclonal antibody (20683-1-AP)

    IHC staining of mouse brain using 20683-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DOCK3; MOCA Polyclonal antibody (20683-1-AP)

    IHC staining of mouse brain using 20683-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, SH-SY5Y cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20683-1-AP targets DOCK3; MOCA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 3
Calculated molecular weight 233 kDa
Observed molecular weight 233 kDa
GenBank accession numberNM_004947
Gene symbol DOCK3
Gene ID (NCBI) 1795
RRIDAB_10696182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dedicator of cytokinesis 3 (DOCK3), also named as MOCA and PBP , is a ~180 kDa protein involved in signaling trasduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3β (GSK-3β) at the plasma membrane, thereby promoteing axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS.

Protocols

Product Specific Protocols
WB protocol for DOCK3; MOCA antibody 20683-1-APDownload protocol
IHC protocol for DOCK3; MOCA antibody 20683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Diabetes

Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.

Authors - Hua Qu
View Article
humanWB

Int J Biochem Cell Biol

Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer.

Authors - Xingli Zhu
View Article