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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DOCK4 Polyclonal antibody

DOCK4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21861-1-AP

Synonyms

KIAA0716, dedicator of cytokinesis 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using DOCK4 Polyclonal antibody (21861-1-AP)

    WB analysis of HEK-293 using 21861-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21861-1-AP (DOCK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DOCK4 Polyclonal antibody (21861-1-AP)

    WB analysis using 21861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21861-1-AP (DOCK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DOCK4 Polyclonal antibody (21861-1-AP)

    IP experiment of HeLa using 21861-1-AP

    IP result of anti-DOCK4 (IP:21861-1-AP, 5ug; Detection:21861-1-AP 1:500) with HeLa cells lysate 1800ug.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using DOCK4 Polyclonal antibody (21861-1-AP)

    IP experiment of HEK-293T using 21861-1-AP

    IP result of anti-DOCK4 (IP:21861-1-AP, 4ug; Detection:21861-1-AP 1:300) with HEK-293T cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human ovary tumor using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human ovary tumor using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human breast cancer using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human breast cancer using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human skeletal muscle using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human skeletal muscle using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human prostate cancer using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DOCK4 Polyclonal antibody (21861-1-AP)

    IHC staining of human prostate cancer using 21861-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK4 Polyclonal antibody (21861-1-AP)

    IF Staining of HeLa using 21861-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21861-1-AP (DOCK4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HEK-293T cells
Positive IP detected inHeLa cells, HEK-293T cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:150-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21861-1-AP targets DOCK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOCK4 fusion protein Ag16516 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 4
Calculated molecular weight 2011 aa, 230 kDa
Observed molecular weight 225 kDa
GenBank accession numberBC117689
Gene symbol DOCK4
Gene ID (NCBI) 9732
RRIDAB_2878930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.

Protocols

Product Specific Protocols
WB protocol for DOCK4 antibody 21861-1-APDownload protocol
IHC protocol for DOCK4 antibody 21861-1-APDownload protocol
IF protocol for DOCK4 antibody 21861-1-APDownload protocol
IP protocol for DOCK4 antibody 21861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum.

Authors - Leah McNally
View Article
humanWB, IHC

Front Cell Dev Biol

USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway.

Authors - Suwei Zhu
View Article
humanWB

Front Oncol

miR-33b-3p Acts as a Tumor Suppressor by Targeting DOCK4 in Prostate Cancer.

Authors - Yu Mei
View Article
humanWB

Science

Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells

Authors - Zhi Lin
View Article