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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using DOCK4 Polyclonal antibody (27151-1-AP)

    WB analysis of HEK-293T using 27151-1-AP (same clone as 27151-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27151-1-AP (DOCK4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27151-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK4 Polyclonal antibody (27151-1-AP)

    IF Staining of HeLa using 27151-1-AP (same clone as 27151-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27151-1-AP (DOCK4 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27151-1-PBS in a different storage buffer formulation.

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DOCK4 Polyclonal antibody, PBS Only

DOCK4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27151-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27151-1-PBS targets DOCK4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOCK4 fusion protein Ag25946 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 4
Calculated molecular weight 2011 aa, 230 kDa
Observed molecular weight 225 kDa
GenBank accession numberBC117689
Gene Symbol DOCK4
Gene ID (NCBI) 9732
RRIDAB_2880776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1I0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.