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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DOHH Polyclonal antibody

DOHH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18226-1-AP

Synonyms

HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOHH Polyclonal antibody (18226-1-AP)

    WB analysis using 18226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18226-1-AP (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inDU 145 cells, LNCaP cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18226-1-AP targets DOHH in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOHH fusion protein Ag13072 相同性解析による交差性が予測される生物種
Full Name deoxyhypusine hydroxylase/monooxygenase
Calculated molecular weight 33 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC002817
Gene symbol DOHH
Gene ID (NCBI) 83475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

eoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

Protocols

Product Specific Protocols
WB protocol for DOHH antibody 18226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols