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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOHH Monoclonal antibody (68151-1-Ig)

    WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DOHH Monoclonal antibody (68151-1-Ig)

    WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using DOHH Monoclonal antibody (68151-1-Ig)

    IF Staining of LNCaP using 68151-1-Ig (same clone as 68151-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using DOHH antibody (68151-1-Ig, Clone: 1H5H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

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DOHH Monoclonal antibody, PBS Only

DOHH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68151-1-PBS
Clone No.1H5H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68151-1-PBS targets DOHH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DOHH fusion protein Ag13041 相同性解析による交差性が予測される生物種
Full Name deoxyhypusine hydroxylase/monooxygenase
Calculated molecular weight 33 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC002817
Gene Symbol DOHH
Gene ID (NCBI) 83475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BU89
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).