Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
14087-1-AP targets DOK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DOK2 fusion protein Ag5218 相同性解析による交差性が予測される生物種 |
| Full Name | docking protein 2, 56kDa |
| Calculated molecular weight | 412 aa, 45 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC032623 |
| Gene Symbol | DOK2 |
| Gene ID (NCBI) | 9046 |
| RRID | AB_2230792 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60496 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region (PMID: 25075100).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DOK2 antibody 14087-1-AP | Download protocol |
| IHC protocol for DOK2 antibody 14087-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Histopathology Expression of T-cell receptor signalling pathway components in extranodal NK/T-cell lymphoma. |