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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPCR1 Polyclonal antibody

DPCR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31420-1-AP

Synonyms

PBLT, MUCL3, C6orf37

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPCR1 Polyclonal antibody (31420-1-AP)

    WB analysis using 31420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31420-1-AP (DPCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DPCR1 Polyclonal antibody (31420-1-AP)

    IHC staining of human stomach cancer using 31420-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31420-1-AP (DPCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using DPCR1 Polyclonal antibody (31420-1-AP)

    IHC staining of mouse stomach using 31420-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31420-1-AP (DPCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inBGC-823 cells, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman stomach cancer tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31420-1-AP targets DPCR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPCR1 fusion protein Ag33318 相同性解析による交差性が予測される生物種
Full Name diffuse panbronchiolitis critical region 1
Calculated molecular weight56 kDa
Observed molecular weight56 kDa
GenBank accession numberNM_080870
Gene symbol DPCR1
Gene ID (NCBI) 135656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Diffuse panbronchiolitis critical region 1 (DPCR1) is located in the major histocompatibility complex (MHC) class I. DPCR1 is downregulated in invasive pituitary adenoma compared with that in non-invasive tumors, but upregulated in the precursor of gastric carcinogenesis (PMID: 29242154).

Protocols

Product Specific Protocols
WB protocol for DPCR1 antibody 31420-1-APDownload protocol
IHC protocol for DPCR1 antibody 31420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols