Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| FC | See 1 publications below |
Product Information
12222-1-AP targets DPEP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2907 Product name: Recombinant human DPEP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-385 aa of BC017023 Sequence: MWSGWWLWPLVAVCTADFFRDEAERIMRDSPVIDGHNDLPWQLLDMFNNRLQDERANLTTLAGTHTNIPKLRAGFVGGQFWSVYTPCDTQNKDAVRRTLEQMDVVHRMCRMYPETFLYVTSSAGIRQAFREGKVASLIGVEGGHSIDSSLGVLRALYQLGMRYLTLTHSCNTPWADNWLVDTGDSEPQSQGLSPFGQRVVKELNRLGVLIDLAHVSVATMKATLQLSRAPVIFSHSSAYSVCASRRNVPDDVLRLVKQTDSLVMVNFYNNYISCTNKANLSQVADHLDHIKEVAGARAVGFGGDFDGVPRVPEGLEDVSKYPDLIAELLRRNWTEAEVKGALADNLLRVFEAVEQASNLTQAPEEEPIPLDQLGGSCRTHYGYSS 相同性解析による交差性が予測される生物種 |
| Full Name | dipeptidase 1 (renal) |
| Calculated molecular weight | 411 aa, 46 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC017023 |
| Gene Symbol | DPEP1 |
| Gene ID (NCBI) | 1800 |
| RRID | AB_2877837 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16444 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DPEP1(Dipeptidase 1) is also named as MDP, RDP and belongs to the peptidase M19 family. It is a glycoprotein that plays an important role in the regulation of leukotriene activity. DPEP1 converts lekotriene D4 to leukotriene E4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DPEP1 antibody 12222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver.
| ||
Sci Adv Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.
| ||
J Clin Invest Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury. | ||
Nat Commun A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
| ||
Int Immunopharmacol Dipeptidase 1 promotes ferroptosis in renal tubular epithelial cells in diabetic nephropathy via inhibition of the GSH/GPX4 axis |
