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Validation Data Gallery

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  • Western Blot (WB) analysis of pig pancreas tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of pig pancreas using 68081-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of human testis using 68081-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of rabbit kidney using 68081-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of rat pancreas using 68081-1-Ig

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of HepG2 using 68081-1-Ig

    WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IHC staining of human colon cancer using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IF Staining of mouse kidney using 68081-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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DPEP1 Monoclonal antibody

DPEP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68081-1-Ig
Clone No.2B4F4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, ELISA

Synonyms

hRDP, EC:3.5.2.6, EC:3.4.13.19, Dehydropeptidase-I, Beta-lactamase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, rabbit kidney tissue, pig pancreas tissue, rat pancreas tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68081-1-Ig targets DPEP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DPEP1 fusion protein Ag14591 相同性解析による交差性が予測される生物種
Full Name dipeptidase 1 (renal)
Calculated molecular weight 411 aa, 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC017023
Gene Symbol DPEP1
Gene ID (NCBI) 1800
RRIDAB_2918818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DPEP1 antibody 68081-1-IgDownload protocol
IHC protocol for DPEP1 antibody 68081-1-IgDownload protocol
IF protocol for DPEP1 antibody 68081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols