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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPEP1 Monoclonal antibody

DPEP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B4F4

Cat no : 68081-1-Ig

Synonyms

hRDP, EC:3.5.2.6, EC:3.4.13.19, Dehydropeptidase-I, Beta-lactamase

Validation Data Gallery

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  • Western Blot (WB) analysis of pig pancreas tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of pig pancreas using 68081-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of human testis using 68081-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of rabbit kidney using 68081-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of rat pancreas using 68081-1-Ig

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DPEP1 Monoclonal antibody (68081-1-Ig)

    WB analysis of HepG2 using 68081-1-Ig

    WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IHC staining of human colon cancer using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using DPEP1 Monoclonal antibody (68081-1-Ig)

    IF Staining of mouse kidney using 68081-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman testis tissue, rabbit kidney tissue, pig pancreas tissue, rat pancreas tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68081-1-Ig targets DPEP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DPEP1 fusion protein Ag14591 相同性解析による交差性が予測される生物種
Full Name dipeptidase 1 (renal)
Calculated molecular weight 411 aa, 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC017023
Gene symbol DPEP1
Gene ID (NCBI) 1800
RRIDAB_2918818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DPEP1 antibody 68081-1-IgDownload protocol
IHC protocol for DPEP1 antibody 68081-1-IgDownload protocol
IF protocol for DPEP1 antibody 68081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols