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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPF1 Polyclonal antibody (21769-1-AP)

    WB analysis using 21769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21769-1-AP (DPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DPF1 Polyclonal antibody (21769-1-AP)

    IF Staining of MCF-7 using 21769-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DPF1 antibody (21769-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

DPF1 Polyclonal antibody

DPF1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21769-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Zinc finger protein neuro-d4, SMARCG1, neuro d4, NEUD4, BRG1-associated factor 45B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, PC-3 cells, mouse cerebellum tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21769-1-AP targets DPF1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPF1 fusion protein Ag16409 相同性解析による交差性が予測される生物種
Full Name D4, zinc and double PHD fingers family 1
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight38-47 kDa
GenBank accession numberBC125153
Gene Symbol DPF1
Gene ID (NCBI) 8193
RRIDAB_3085672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92782
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DPF1 antibody 21769-1-APDownload protocol
IF protocol for DPF1 antibody 21769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols