DPP4/CD26 Polyclonal antibody
DPP4/CD26 Polyclonal Antibody for WB, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29403-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Caco-2 cells, HT-29 cells |
Positive FC detected in | HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
29403-1-AP targets DPP4/CD26 in WB, FC, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DPP4/CD26 fusion protein Ag30160 相同性解析による交差性が予測される生物種 |
Full Name | dipeptidyl-peptidase 4 |
Calculated molecular weight | 88 kDa |
Observed molecular weight | 110 kDa |
GenBank accession number | BC013329 |
Gene symbol | DPP4 |
Gene ID (NCBI) | 1803 |
RRID | AB_2918296 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).
Protocols
Product Specific Protocols | |
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WB protocol for DPP4/CD26 antibody 29403-1-AP | Download protocol |
FC protocol for DPP4/CD26 antibody 29403-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Pharmacother Fructus Lycii and Salvia miltiorrhiza Bunge extract attenuate oxidative stress-induced photoreceptor ferroptosis in retinitis pigmentosa | ||
J Transl Med Characterization of the proteome of stable and unstable carotid atherosclerotic plaques using data-independent acquisition mass spectrometry | ||
Anal Chem Ultrasensitive Fluorescent Probes Reveal an Adverse Action of Dipeptide Peptidase IV and Fibroblast Activation Protein during Proliferation of Cancer Cells |