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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPP6 Polyclonal antibody

DPP6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28143-1-AP

Synonyms

DPP VI, Dipeptidyl peptidase VI, Dipeptidyl peptidase IV-like protein, Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-related protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP6 Polyclonal antibody (28143-1-AP)

    WB analysis using 28143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28143-1-AP (DPP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DPP6 Polyclonal antibody (28143-1-AP)

    IP experiment of mouse brain using 28143-1-AP

    IP result of anti-DPP6 (IP:28143-1-AP, 4ug; Detection:28143-1-AP 1:4000) with mouse brain tissue lysate 1840 ug.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using DPP6 Polyclonal antibody (28143-1-AP)

    IF Staining of SH-SY5Y using 28143-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DPP6 antibody (28143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28143-1-AP targets DPP6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPP6 fusion protein Ag27338 相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 6
Observed molecular weight 98-101 kDa
GenBank accession numberBC150304
Gene symbol DPP6
Gene ID (NCBI) 1804
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DPP6 (Dipeptidyl aminopeptidase-like protein 6) is also named as DPPX and Dipeptidyl peptidase VI (DPP VI). DPP6 is a type II transmembrane protein, and DPP6 is a multifunctional protein with considerable impact on brain development and function (PMID: 35395074). In breast cancer tissues, DPP6 has low expression at the transcription and protein levels, and DPP6 may serve as a tumour suppressor in tumour development (PMID: 36531033).

Protocols

Product Specific Protocols
WB protocol for DPP6 antibody 28143-1-APDownload protocol
IF protocol for DPP6 antibody 28143-1-APDownload protocol
IP protocol for DPP6 antibody 28143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols