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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPP7 Polyclonal antibody

DPP7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19018-1-AP

Synonyms

DPP2, DPP II, Dipeptidyl peptidase II, Dipeptidyl peptidase 7, Dipeptidyl peptidase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP7 Polyclonal antibody (19018-1-AP)

    WB analysis using 19018-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19018-1-AP (DPP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DPP7 Polyclonal antibody (19018-1-AP)

    IHC staining of human ovary cancer using 19018-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19018-1-AP (DPP7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inU-937 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19018-1-AP targets DPP7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPP7 fusion protein Ag13556 相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 7
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC016961
Gene symbol DPP7
Gene ID (NCBI) 29952
RRIDAB_10641993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DPP7 (also known as DPP-II and quiescent cell proline dipeptidase, QPP), is the first reported protease that contains a leucine zipper motif through which the functional homodimer has been predicted to be formed (PMID: 22952628). DPP7 can be detected as 54 kDa (full length) and ~50 kDa (signal peptide cleaved).

Protocols

Product Specific Protocols
WB protocol for DPP7 antibody 19018-1-APDownload protocol
IHC protocol for DPP7 antibody 19018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines.

Authors - Alexander Schepsky
View Article