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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPPA2 Polyclonal antibody, PBS Only

DPPA2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12689-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of MCF-7 using 12689-1-AP (same clone as 12689-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of HepG2 using 12689-1-AP (same clone as 12689-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of HepG2 using 12689-1-AP (same clone as 12689-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of MCF-7 using 12689-1-AP (same clone as 12689-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12689-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    IF Staining of A431 using 12689-1-AP (same clone as 12689-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 12689-1-AP (DPPA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12689-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12689-1-PBS targets DPPA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPPA2 fusion protein Ag3601 相同性解析による交差性が予測される生物種
Full Name developmental pluripotency associated 2
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC018070
Gene symbol DPPA2
Gene ID (NCBI) 151871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.