CRMP5 Polyclonal antibody
CRMP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10525-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
10525-1-AP targets CRMP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CRMP5 fusion protein Ag0783 相同性解析による交差性が予測される生物種 |
| Full Name | dihydropyrimidinase-like 5 |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC002874 |
| Gene symbol | CRMP5 |
| Gene ID (NCBI) | 56896 |
| RRID | AB_2094644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DPYSL5(Dihydropyrimidinase-related protein 5) is also named as CRMP5, ULIP6 and belongs to the hydantoinase/dihydropyrimidinase subfamily. It may have a function in neuronal differentiation and/or axon growth. SCLCs universally express CRMP5 and in thymoma, none of the CRMPs are detected by immunohistochemistry in tumorous epithelial cells with specific antibodies including CRMP5.The normal CRMP5 is probably not expressed by tumorous epithelial cells.(PMID:16519949).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| IHC protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| IF protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| IP protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Int Med Res Ubiquitin C-terminal hydrolase L1 promotes lymph node metastasis in small cell neuroendocrine carcinomas of the cervix. |
