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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using DR5 Recombinant antibody (84524-4-RR)

    WB analysis of NIH/3T3 using 84524-4-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84524-4-RR (DR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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DR5 Recombinant antibody

DR5 Recombinant Antibody for WB, ELISA
Cat No. 84524-4-RR
Clone No.241847B8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Tnfrsf10b, MK, Killer, Death receptor 5, CD262

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84524-4-RR targets DR5 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 10b
Calculated molecular weight42kDa
Observed molecular weight50 kDa
GenBank accession numberNM_020275.4
Gene Symbol DR5
Gene ID (NCBI) 21933
RRIDAB_3672032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9QZM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DR5, also known as CD262, TNFRSF10B, TRAILR2, TRICK2 and KILLER, is a widely expressed single-pass type I membrane protein belonging to the tumour necrosis factor receptor superfamily (TNFRSF). It is a receptor for TNF-related apoptosis-inducing ligand (TRAIL), which is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells (PMID: 9311998). DR5 contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain (DD), through which DR5 is capable to transmit the apoptotic signal (PMID: 9311998; 20531300).

Protocols

Product Specific Protocols
WB protocol for DR5 antibody 84524-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols