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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DRAP1 Polyclonal antibody

DRAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11020-1-AP

Synonyms

NC2-alpha, NC2 alpha, Dr1-associated protein 1, Dr1-associated corepressor, Dr1 associated protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DRAP1 Polyclonal antibody (11020-1-AP)

    WB analysis using 11020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11020-1-AP (DRAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using DRAP1 Polyclonal antibody (11020-1-AP)

    WB analysis of human heart using 11020-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11020-1-AP (DRAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DRAP1 Polyclonal antibody (11020-1-AP)

    WB analysis of human brain using 11020-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11020-1-AP (DRAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using DRAP1 Polyclonal antibody (11020-1-AP)

    WB analysis of mouse kidney using 11020-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11020-1-AP (DRAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DRAP1 Polyclonal antibody (11020-1-AP)

    IHC staining of human pancreas cancer using 11020-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11020-1-AP (DRAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DRAP1 Polyclonal antibody (11020-1-AP)

    IF Staining of HepG2 using 11020-1-AP

    Immunofluorescent analysis of HepG2 cells, using DRAP1 antibody 11020-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inmouse testis tissue, human heart tissue, human brain tissue, mouse kidney tissue, rat testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11020-1-AP targets DRAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DRAP1 fusion protein Ag1470 相同性解析による交差性が予測される生物種
Full Name DR1-associated protein 1 (negative cofactor 2 alpha)
Calculated molecular weight 22 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC010025
Gene symbol DRAP1
Gene ID (NCBI) 10589
RRIDAB_2277501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DRAP1 antibody 11020-1-APDownload protocol
IHC protocol for DRAP1 antibody 11020-1-APDownload protocol
IF protocol for DRAP1 antibody 11020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols