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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DRC1 Polyclonal antibody (26219-1-AP)

    WB analysis using 26219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26219-1-AP (DRC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DRC1 Polyclonal antibody (26219-1-AP)

    IF Staining of hTERT-RPE1 using 26219-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DRC1 antibody (26219-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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DRC1 Polyclonal antibody

DRC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26219-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

C2orf39, Dynein regulatory complex protein 1, Coiled-coil domain-containing protein 164, CCDC164

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26219-1-AP targets DRC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DRC1 fusion protein Ag24113 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 39
Observed molecular weight 87 kDa
GenBank accession numberBC009488
Gene Symbol C2orf39/DRC1
Gene ID (NCBI) 92749
RRIDAB_3085848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MC2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DRC1, also known as C2orf39, CCDC164, has 3 coiled-coil motifs in its N-terminal half and an additional coiled-coil motif near its C terminus. DRC1 is a subunit of the nexin-dynein regulatory complex, an axonemal structure critical for the regulation of ciliary dynein motors. DRC1 is predominantly expressed in the lung, brain, and prostate (PMID: 23354437). DRC1 deficiency disrupts the assembly of the nexin-dynein regulatory complex, causing ciliary dyskinesia(PMID: 23354437, 34815526).

Protocols

Product Specific Protocols
WB protocol for DRC1 antibody 26219-1-APDownload protocol
IF protocol for DRC1 antibody 26219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols