Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 26 publications below |
| IHC | See 5 publications below |
| IF | See 6 publications below |
Product Information
17934-1-AP targets DRD1 in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12366 Product name: Recombinant human DRD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 338-446 aa of BC074978 Sequence: RKAFSTLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT 相同性解析による交差性が予測される生物種 |
| Full Name | dopamine receptor D1 |
| Calculated molecular weight | 446 aa, 49 kDa |
| Observed molecular weight | 50-75 kDa |
| GenBank accession number | BC074978 |
| Gene Symbol | DRD1 |
| Gene ID (NCBI) | 1812 |
| RRID | AB_10598308 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21728 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Dopamine is a neurotransmitter that plays a crucial role in physical and mental health, such as cardiovascular, hormonal, renal, and central nervous systems (PMID: 29220802). Five subtypes of mammalian dopamine receptors are grouped into two classes, the D1- and D2-like classes. The D1-like class includes D1 and D5 receptors whereas the D2-like class includes D2, D3, D4 subtypes (PMID: 9457173). Dopamine receptor D1 (DRD1) is the most abundant form of dopamine receptor in the central nervous system. DRD1 stimulates adenylate cyclase, modulates D2 receptor activity, regulates neuron growth and differentiation, and mediates several behavioral responses (PMID: 1977312). DRD1 has a calculated molecular weight of 49 kDa, larger apparent molecular weight of 60-80 kDa may be due to glycosylation (PMID: 1281547; 23821371).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DRD1 antibody 17934-1-AP | Download protocol |
| WB protocol for DRD1 antibody 17934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunity Dopamine inhibits group 2 innate lymphoid cell-driven allergic lung inflammation by dampening mitochondrial activity | ||
Adv Mater Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects | ||
Mol Neurodegener Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency | ||
Nat Commun Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis | ||
J Adv Res The heterogeneity of dopamine-mediated vasodilation in human intrarenal arteries. |