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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DROSHA Polyclonal antibody (27958-1-AP)

    WB analysis using 27958-1-AP (same clone as 27958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27958-1-AP (DROSHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DROSHA Polyclonal antibody (27958-1-AP)

    WB analysis of HEK-293 using 27958-1-AP (same clone as 27958-1-PBS)

    WB result of DROSHA antibody (27958-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DROSHA transfected HEK-293 cells. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DROSHA Polyclonal antibody (27958-1-AP)

    IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DROSHA Polyclonal antibody (27958-1-AP)

    IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DROSHA Polyclonal antibody, PBS Only

DROSHA Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27958-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RNASEN, RANSE3L, Protein Drosha, p241, HSA242976

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27958-1-PBS targets DROSHA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DROSHA fusion protein Ag27533 相同性解析による交差性が予測される生物種
Full Name ribonuclease type III, nuclear
Calculated molecular weight 159 aa
Observed molecular weight150 kDa
GenBank accession numberNM_001100412
Gene Symbol DROSHA
Gene ID (NCBI) 29102
RRIDAB_2918137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRR4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.