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DRP1 (C-terminal) Recombinant antibody

DRP1 (C-terminal) Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A19

Cat no : 81561-1-RR

Synonyms

DRP1, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L, DLP1


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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, A549 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81561-1-RR targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DRP1 (C-terminal) fusion protein Ag3644 相同性解析による交差性が予測される生物種
Full Name dynamin 1-like
Calculated molecular weight 736 aa, 82 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC024590
Gene symbol DRP1
Gene ID (NCBI) 10059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
IHC protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
IF protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
FC protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols