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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DRP1 (C-terminal) Recombinant antibody (81561-1-RR)

    WB analysis using 81561-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DRP1 (C-terminal) Recombinant antibody (81561-1-RR)

    WB analysis of HEK-293 using 81561-1-RR

    WB result of DRP1 (C-terminal) antibody (81561-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using DRP1 (C-terminal) Recombinant antibody (81561-1-RR)

    WB analysis using 81561-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DRP1 (C-terminal) Recombinant antibody (81561-1-RR)

    IHC staining of mouse cerebellum using 81561-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DRP1 (C-terminal) Recombinant antibody (81561-1-RR)

    IHC staining of mouse cerebellum using 81561-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using DRP1 (C-terminal) Recombinant antibody (81561-1-RR)

    IF Staining of NIH/3T3 using 81561-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DRP1 (C-terminal) antibody (81561-1-RR, Clone: 2A19 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using DRP1 (C-terminal) Recombinant antibody (81561-1-RR)

    FC experiment of HeLa using 81561-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (81561-1-RR, Clone:2A19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 81561-1-RR

    Affinity and Kinetic Characterization of 81561-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81561-1-RR against Human DRP1 (C-terminal) were performed. The affinity constant is 0.825 nM.

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  • Featured Product
  • KD/KO Validated

DRP1 (C-terminal) Recombinant antibody

DRP1 (C-terminal) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 81561-1-RR
Clone No.2A19

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

DRP1, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L, DLP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, A549 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81561-1-RR targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DRP1 (C-terminal) fusion protein Ag3644 相同性解析による交差性が予測される生物種
Full Name dynamin 1-like
Calculated molecular weight 736 aa, 82 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC024590
Gene Symbol DRP1
Gene ID (NCBI) 10059
RRIDAB_2935561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
IHC protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
IF protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
FC protocol for DRP1 (C-terminal) antibody 81561-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols