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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DSE Polyclonal antibody (10452-1-AP)

    IHC staining of human ovary tumor using 10452-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10452-1-AP (DSE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DSE Polyclonal antibody (10452-1-AP)

    IHC staining of human ovary tumor using 10452-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10452-1-AP (DSE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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DSE Polyclonal antibody

DSE Polyclonal Antibody for IHC, ELISA
Cat No. 10452-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Synonyms

dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10452-1-AP targets DSE in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DSE fusion protein Ag0695 相同性解析による交差性が予測される生物種
Full Name dermatan sulfate epimerase
Calculated molecular weight 958 aa, 110 kDa
GenBank accession numberBC039245
Gene Symbol DSE
Gene ID (NCBI) 29940
RRIDAB_2093288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

Protocols

Product Specific Protocols
IHC protocol for DSE antibody 10452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC, IF

Liver Int

Expression of chondroitin-glucuronate C5-epimerase and cellular immune responses in patients with hepatocellular carcinoma.

Authors - Mizukoshi Eishiro E
View Article