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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using DSE Monoclonal antibody (60041-1-Ig)

    WB analysis of COLO 320 using 60041-1-Ig (same clone as 60041-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60041-1-Ig (DSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DSE Monoclonal antibody (60041-1-Ig)

    IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DSE Monoclonal antibody (60041-1-Ig)

    IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DSE Monoclonal antibody (60041-1-Ig)

    IF Staining of HeLa using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSE antibody (60041-1-Ig, Clone: 2C9F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

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DSE Monoclonal antibody, PBS Only

DSE Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60041-1-PBS
Clone No.2C9F7

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60041-1-PBS targets DSE in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen DSE fusion protein Ag0695 相同性解析による交差性が予測される生物種
Full Name dermatan sulfate epimerase
Calculated molecular weight 958 aa, 110 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC039245
Gene Symbol DSE
Gene ID (NCBI) 29940
RRIDAB_2093286
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ9UL01
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.