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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DSP Monoclonal antibody, PBS Only

DSP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10D4

Cat no : 68364-1-PBS

Synonyms

desmoplakin, DP, DPI, DPII, DSP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSP Monoclonal antibody (68364-1-Ig)

    WB analysis using 68364-1-Ig (same clone as 68364-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DSP Monoclonal antibody (68364-1-Ig)

    IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DSP Monoclonal antibody (68364-1-Ig)

    IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DSP Monoclonal antibody (68364-1-Ig)

    IF Staining of A431 using 68364-1-Ig (same clone as 68364-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68364-1-PBS targets DSP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DSP fusion protein Ag30499 相同性解析による交差性が予測される生物種
Full Name desmoplakin
Calculated molecular weight 2871 aa, 332 kDa
Observed molecular weight 240-330 kDa
GenBank accession numberBC140802
Gene symbol DSP
Gene ID (NCBI) 1832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)