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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DSPP Polyclonal antibody, PBS Only

DSPP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55396-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSPP Polyclonal antibody (55396-1-AP)

    WB analysis using 55396-1-AP (same clone as 55396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55396-1-AP (DSPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55396-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DSPP Polyclonal antibody (55396-1-AP)

    IF Staining of HeLa using 55396-1-AP (same clone as 55396-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55396-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DSPP Polyclonal antibody (55396-1-AP)

    IF Staining of HeLa using 55396-1-AP (same clone as 55396-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55396-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55396-1-PBS targets DSPP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name dentin sialophosphoprotein
Calculated molecular weight 131 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberNM_014208
Gene symbol DSPP
Gene ID (NCBI) 1834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. The molecular mass of full-length is around 130kDa(uniprot), the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).