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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DST Polyclonal antibody

DST Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28438-1-AP

Synonyms

BP240, BPA, BPAG1, Bullous pemphigoid antigen, Bullous pemphigoid antigen 1, CATX 15, D6S1101, DKFZp564B2416, DMH, DST, DT, Dystonia musculorum protein, dystonin, FLJ46791, Hemidesmosomal plaque protein, KIAA0465, KIAA0728, KIAA1470, MACF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DST Polyclonal antibody (28438-1-AP)

    WB analysis using 28438-1-AP

    Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DST Polyclonal antibody (28438-1-AP)

    WB analysis using 28438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DST Polyclonal antibody (28438-1-AP)

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DST Polyclonal antibody (28438-1-AP)

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DST Polyclonal antibody (28438-1-AP)

    IHC staining of human liver cancer using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DST Polyclonal antibody (28438-1-AP)

    IHC staining of human liver cancer using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using DST Polyclonal antibody (28438-1-AP)

    IF Staining of SH-SY5Y using 28438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 28438-1-AP (DST antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28438-1-AP targets DST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DST fusion protein Ag28833 相同性解析による交差性が予測される生物種
Full Name dystonin
Calculated molecular weight 861 kDa
GenBank accession numberNM_001144769
Gene symbol DST
Gene ID (NCBI) 667
RRIDAB_2881141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DST antibody 28438-1-APDownload protocol
IHC protocol for DST antibody 28438-1-APDownload protocol
IF protocol for DST antibody 28438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols