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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Destrin Polyclonal antibody

Destrin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10962-2-AP

Synonyms

DSTN, DSN, ADF, Actin-depolymerizing factor, ACTDP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Destrin Polyclonal antibody (10962-2-AP)

    WB analysis using 10962-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10962-2-AP (Destrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Destrin Polyclonal antibody (10962-2-AP)

    IHC staining of human skin cancer using 10962-2-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10962-2-AP (Destrin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Destrin Polyclonal antibody (10962-2-AP)

    IF Staining of MCF-7 using 10962-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Destrin antibody (10962-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, PC-3 cells, SKOV-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10962-2-AP targets Destrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Destrin fusion protein Ag1402 相同性解析による交差性が予測される生物種
Full Name destrin (actin depolymerizing factor)
Calculated molecular weight 19 kDa
Observed molecular weight18 kDa
GenBank accession numberBC009477
Gene symbol Destrin
Gene ID (NCBI) 11034
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.

Protocols

Product Specific Protocols
WB protocol for Destrin antibody 10962-2-APDownload protocol
IHC protocol for Destrin antibody 10962-2-APDownload protocol
IF protocol for Destrin antibody 10962-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols