Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
20102-1-AP targets DSTYK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DSTYK fusion protein Ag13678 相同性解析による交差性が予測される生物種 |
| Full Name | dual serine/threonine and tyrosine protein kinase |
| Calculated molecular weight | 66 kDa, 105 kDa |
| Observed molecular weight | 66 kDa, 100~105 kDa |
| GenBank accession number | BC060870 |
| Gene Symbol | DSTYK |
| Gene ID (NCBI) | 25778 |
| RRID | AB_10694146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6XUX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DSTYK (Dual serine-threonine and tyrosine protein kinase gene), also known as CAKUT1, DustyPK, SPG23 has been classically considered as RIPK5, encodes a protein product consisting of 850-931 amino acids, which has a striking membrane-associated distribution in mesenchymal-derived cells of all major organs including brain, heart, kidney, lung, colon, and muscle (PMID: 34853310). DSTYK is strongly expressed in the cytoplasm of most malignant cells and that the high expression of DSTYK in human colon cancer specimens is associated with tumor progression and/or poor prognosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DSTYK antibody 20102-1-AP | Download protocol |
| IF protocol for DSTYK antibody 20102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. | ||
Cell Death Dis Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma
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