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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DTD1 Polyclonal antibody

DTD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17480-1-AP

Synonyms

C20orf88, DTD1, DUEB, HARS2, Histidyl tRNA synthase related, pqn 68

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DTD1 Polyclonal antibody (17480-1-AP)

    WB analysis using 17480-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17480-1-AP (DTD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using DTD1 Polyclonal antibody (17480-1-AP)

    IF Staining of Jurkat using 17480-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DTD1 antibody (17480-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DTD1 Polyclonal antibody (17480-1-AP)

    IF Staining of A549 using 17480-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 17480-1-AP (DTD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IF/ICC detected inJurkat cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17480-1-AP targets DTD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTD1 fusion protein Ag11510 相同性解析による交差性が予測される生物種
Full Name D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae)
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC045167
Gene symbol DTD1
Gene ID (NCBI) 92675
RRIDAB_2230675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DTD1 antibody 17480-1-APDownload protocol
IF protocol for DTD1 antibody 17480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols