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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DTNB Polyclonal antibody

DTNB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12045-1-AP

Synonyms

Beta dystrobrevin, DTN B, DTNB, Dystrobrevin beta, dystrobrevin, beta

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DTNB Polyclonal antibody (12045-1-AP)

    WB analysis of mouse skeletal muscle using 12045-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12045-1-AP (DTNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using DTNB Polyclonal antibody (12045-1-AP)

    WB analysis of mouse liver using 12045-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12045-1-AP (DTNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DTNB Polyclonal antibody (12045-1-AP)

    WB analysis of NIH/3T3 using 12045-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12045-1-AP (DTNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DTNB Polyclonal antibody (12045-1-AP)

    IHC staining of human kidney using 12045-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12045-1-AP (DTNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse liver tissue, NIH/3T3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12045-1-AP targets DTNB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTNB fusion protein Ag2675 相同性解析による交差性が予測される生物種
Full Name dystrobrevin, beta
Calculated molecular weight 560 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC016655
Gene symbol DTNB
Gene ID (NCBI) 1838
RRIDAB_2093901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DTNB antibody 12045-1-APDownload protocol
IHC protocol for DTNB antibody 12045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols