Validation Data Gallery

Filter: AllWBIHC

WB analysis using 25304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of hTERT-RPE1 using 25304-1-AP
Western blot analysis in wildtype and DTX3 knockouted human hTERT-RPE1 cells with 25304-1-AP (DTX3 antibody). The data provided by an anonymous customer.
WB analysis using 25304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 25304-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 25304-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 25304-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 25304-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 25304-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 25304-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 25304-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 10x lens).

Featured Product
KD/KO Validated

DTX3 Polyclonal antibody

DTX3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25304-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

deltex homolog 3 (Drosophila), Deltex3, DTX3, Protein deltex 3, RING finger protein 154, RNF154

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse brain tissue, hTERT-RPE1 cells, rat testis tissue
Positive IHC detected in	human kidney tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below

Product Information

25304-1-AP targets DTX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DTX3 fusion protein Ag18111 相同性解析による交差性が予測される生物種
Full Name	deltex homolog 3 (Drosophila)
Calculated molecular weight	347 aa, 38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC114498
Gene Symbol	DTX3
Gene ID (NCBI)	196403
RRID	AB_2880021
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N9I9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.

Protocols

Product Specific Protocols
WB protocol for DTX3 antibody 25304-1-AP	Download protocol
IHC protocol for DTX3 antibody 25304-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cancer Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway. Authors - Lidong Wang KD Validated View Article
human	WB	Mol Biol Rep Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1. Authors - Hongli Xu KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 25304-1-AP

WB analysis of hTERT-RPE1 using 25304-1-AP

WB analysis using 25304-1-AP

WB analysis of mouse testis using 25304-1-AP

WB analysis of mouse brain using 25304-1-AP

WB analysis of mouse kidney using 25304-1-AP

IHC staining of human kidney using 25304-1-AP

IHC staining of human colon using 25304-1-AP