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DTX3 Polyclonal antibody
DTX3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25304-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse brain tissue, hTERT-RPE1 cells, rat testis tissue |
Positive IHC detected in | human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 2 publications below |
Product Information
25304-1-AP targets DTX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DTX3 fusion protein Ag18111 相同性解析による交差性が予測される生物種 |
Full Name | deltex homolog 3 (Drosophila) |
Calculated molecular weight | 347 aa, 38 kDa |
Observed molecular weight | 38 kDa |
GenBank accession number | BC114498 |
Gene symbol | DTX3 |
Gene ID (NCBI) | 196403 |
RRID | AB_2880021 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.
Protocols
Product Specific Protocols | |
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WB protocol for DTX3 antibody 25304-1-AP | Download protocol |
IHC protocol for DTX3 antibody 25304-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cancer Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.
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Mol Biol Rep Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1.
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