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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DTX3L Polyclonal antibody (11963-1-AP)

    WB analysis of MCF-7 using 11963-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11963-1-AP (DTX3L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using DTX3L Polyclonal antibody (11963-1-AP)

    WB analysis of U2OS using 11963-1-AP

    WB result of DTX3L antibody (11963-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DTX3L transfected U2OS cells.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of human ovary cancer using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of human tonsillitis using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of mouse lung using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of mouse ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of rat kidney using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of rat lung using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of rat ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of mouse ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of rat ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DTX3L Polyclonal antibody (11963-1-AP)

    IHC staining of human ovary cancer using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • KD/KO Validated

DTX3L Polyclonal antibody

DTX3L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11963-1-AP
Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

BBAP, B-lymphoma- and BAL-associated protein, deltex 3 like (Drosophila), E3 ubiquitin-protein ligase DTX3L, EC:2.3.2.27

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, U2OS cells
Positive IHC detected inhuman ovary cancer tissue, mouse lung tissue, rat kidney tissue, rat ovary tissue, mouse ovary tissue, rat lung tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11963-1-AP targets DTX3L in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2568

Product name: Recombinant human DTX3L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 430-741 aa of BC060509

Sequence: SVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESMTLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGICVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESFGTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDVITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE

相同性解析による交差性が予測される生物種
Full Name deltex 3-like (Drosophila)
Calculated molecular weight 740 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC060509
Gene Symbol DTX3L
Gene ID (NCBI) 151636
RRIDAB_874119
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for DTX3L antibody 11963-1-APDownload protocol
WB protocol for DTX3L antibody 11963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Biol Macromol

E3 ubiquitin ligase DTX3L promotes breast cancer progression by enhancing PKCα ubiquitination and inhibiting the p38 MAPK signaling pathway.

Authors - Xiaoxiao Zhang
View Article
humanWB

Cancer Cell Int

Circular RNA circEYA3 promotes the radiation resistance of hepatocellular carcinoma via the IGF2BP2/DTX3L axis

Authors - Pan Hu
View Article
goatIHC

Biomolecules

Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats.

Authors - Xupeng Zang
View Article
humanWB

Sci Rep

DTX3L promotes ovarian cancer progression and cisplatin resistance by activating JAK1/STAT1/OAS3 pathway.

Authors - Fangfang Lu
  • KD Validated
View Article
humanWB

Cancer Sci

Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.

Authors - Xin-Yu Ding
View Article
humanWB,IHC

Sci Rep

Pan-cancer analysis of DTX3L as a potential prognostic and immunological biomarker.

Authors - Yunfeng Zhang
  • KD Validated
View Article
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