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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using DUOXA1 Polyclonal antibody (26632-1-AP)

    WB analysis of mouse colon using 26632-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 26632-1-AP (DUOXA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DUOXA1 Polyclonal antibody (26632-1-AP)

    WB analysis of Jurkat using 26632-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26632-1-AP (DUOXA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using DUOXA1 Polyclonal antibody (26632-1-AP)

    WB analysis of mouse brain using 26632-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26632-1-AP (DUOXA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

DUOXA1 Polyclonal antibody

DUOXA1 Polyclonal Antibody for WB, ELISA
Cat No. 26632-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Dual oxidase activator 1, DUOXA1, mol, NIP, Numb interacting protein, NUMBIP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, Jurkat cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26632-1-AP targets DUOXA1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUOXA1 fusion protein Ag24373 相同性解析による交差性が予測される生物種
Full Name dual oxidase maturation factor 1
Calculated molecular weight 38 kDa
Observed molecular weight 29 kDa, 52 kDa
GenBank accession numberBC020841
Gene Symbol DUOXA1
Gene ID (NCBI) 90527
RRIDAB_2880581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1HG43
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DUOXA1, also named as NIP and NUMBIP, originally identified as a Numb-interacting protein, was recently shown to function as a maturation factor for the dual oxidase 1(DUOX1). DUOXA1 transient overexpression affected the cell-cell adhesion by modulating the actin cytoskeleton, and sensitized cells to doxorubicin. DUOXA1 has some isoforms with MW 33 kDa, 38 kDa and 54 kDa.

Protocols

Product Specific Protocols
WB protocol for DUOXA1 antibody 26632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

PMID: 30166592

Authors - Jing Sun
  • KD Validated
View Article