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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUPD1 / DUSP27 Polyclonal antibody (25259-1-AP)

    WB analysis using 25259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25259-1-AP (DUPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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DUPD1 / DUSP27 Polyclonal antibody

DUPD1 / DUSP27 Polyclonal Antibody for WB, ELISA
Cat No. 25259-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, ELISA

Synonyms

DUPD1, DUSP27, FMDSP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25259-1-AP targets DUPD1 / DUSP27 in WB, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUPD1 / DUSP27 fusion protein Ag19658 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase and pro isomerase domain containing 1
Calculated molecular weight 220 aa, 25 kDa
Observed molecular weight27 kDa
GenBank accession numberBC137322
Gene Symbol DUSP27
Gene ID (NCBI) 338599
RRIDAB_3085779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68J44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dupd1also named as Dusp27, has been categorized as an atypical dual-specificity phosphatase (Dusp) capable of dephosphorylating both tyrosine and serine/threonine residues. It's involved in the modulation of intracellular signaling cascades. In skeletal muscle Dupd1 regulates systemic glucose homeostasis by activating AMPK, which is an energy sensor protein kinase. Dupd1 also affects MAP kinase signaling though modulation of the MAPK1/2 cascade in skeletal muscle promoting muscle cell differentiation, development and atrophy.

Protocols

Product Specific Protocols
WB protocol for DUPD1 / DUSP27 antibody 25259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols