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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DUS2L Recombinant antibody

DUS2L Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240192C6

Cat no : 83265-8-RR

Synonyms

hDUS2, FLJ20399, EC:1.3.1.91, DUS2, Dihydrouridine synthase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUS2L Recombinant antibody (83265-8-RR)

    WB analysis using 83265-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83265-8-RR (DUS2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using DUS2L Recombinant antibody (83265-8-RR)

    FC experiment of HeLa using 83265-8-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUS2L Recombinant antibody (83265-8-RR, Clone:240192C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83265-8-RR

    Affinity and Kinetic Characterization of 83265-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83265-8-RR against Human DUS2L were performed. The affinity constant is 0.321 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, RAW 264.7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83265-8-RR is DUS2L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DUS2L fusion protein Ag23831 相同性解析による交差性が予測される生物種
Full Name dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
Observed molecular weight 55 kDa
GenBank accession numberBC006527
Gene symbol DUS2L
Gene ID (NCBI) 54920
RRIDAB_3670938
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dihydrouridine synthase 2(DUS2L), is a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. transfection of DUS2 cDNA into a human lung carcinoma cell line increased dihydrouridine in tRNA, whereas transfection of DUS2 small interfering RNA (siRNA) reduced tRNA-dihydrouridine, confirming that DUS2 has dihydrouridine synthase activity.

Protocols

Product Specific Protocols
WB protocol for DUS2L antibody 83265-8-RRDownload protocol
FC protocol for DUS2L antibody 83265-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols