DUS4L Polyclonal antibody

DUS4L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21870-1-AP

Synonyms

DUS4, DUS4L, PP35


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Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21870-1-AP is DUS4L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUS4L fusion protein Ag16145 相同性解析による交差性が予測される生物種
Full Name dihydrouridine synthase 4-like (S. cerevisiae)
Calculated molecular weight 317 aa, 36 kDa
Observed molecular weight 22 kDa, 30 kDa
GenBank accession numberBC111774
Gene symbol DUS4L
Gene ID (NCBI) 11062
RRIDAB_2878933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

tRNA-dihydrouridine synthase 4-like belongs to the dus family.It catalyzes the synthesis of dihydrouridine and a modified base found in the D-loop of most tRNAs.Researches showed that DUS4L was alsophosphorylated in vitro by a Ca2+ calmodulin-activated kinase. It has 2 isoforms produced by alternative splicing .One, at 35 kDa, comigrated with a second cAMP-stimulated RIS-ROS phosphoprotein,DUS4L,which was also detected in the cytoskeleton.This antibody recognize the C-terminal of DUS4L.

Protocols

Product Specific Protocols
WB protocol for DUS4L antibody 21870-1-APDownload protocol
IHC protocol for DUS4L antibody 21870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols