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Species Reactivity

Species Reactivity

Conjugate

Conjugate

DUSP13 Polyclonal antibody

DUSP13 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10909-1-AP

Synonyms

MDSP, EC:3.1.3.16, DUSP13B, DUSP13A, Dual specificity protein phosphatase 13B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP13 Polyclonal antibody (10909-1-AP)

    WB analysis using 10909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10909-1-AP (DUSP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using DUSP13 Polyclonal antibody (10909-1-AP)

    WB analysis of human skeletal muscle using 10909-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10909-1-AP (DUSP13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DUSP13 Polyclonal antibody (10909-1-AP)

    WB analysis of mouse testis using 10909-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10909-1-AP (DUSP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DUSP13 Polyclonal antibody (10909-1-AP)

    IP experiment of mouse testis using 10909-1-AP

    IP result of anti-DUSP13 (IP:10909-1-AP, 4ug; Detection:10909-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DUSP13 Polyclonal antibody (10909-1-AP)

    IHC staining of human colon cancer using 10909-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10909-1-AP (DUSP13 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DUSP13 Polyclonal antibody (10909-1-AP)

    IHC staining of human colon cancer using 10909-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10909-1-AP (DUSP13 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DUSP13 Polyclonal antibody (10909-1-AP)

    IHC staining of human colon cancer using 10909-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10909-1-AP (DUSP13 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DUSP13 Polyclonal antibody (10909-1-AP)

    IHC staining of human colon cancer using 10909-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10909-1-AP (DUSP13 antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

Positive WB detected inmouse testis tissue, human skeletal muscle tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10909-1-AP targets DUSP13 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP13 fusion protein Ag1348 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 13
Calculated molecular weight 22 kDa, 36 kDa
Observed molecular weight 22 kDa, 36 kDa
GenBank accession numberBC009778
Gene symbol DUSP13
Gene ID (NCBI) 51207
RRIDAB_2230734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DUSP13 antibody 10909-1-APDownload protocol
IHC protocol for DUSP13 antibody 10909-1-APDownload protocol
IP protocol for DUSP13 antibody 10909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomedicines

The Strong Activation of p53 Tumor Suppressor Drives the Synthesis of the Enigmatic Isoform of DUSP13 Protein

Authors - Małgorzata Krześniak
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Agnieszka (Verified Customer) (01-13-2023)

Product observed at the correct height

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000