Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| FC | See 1 publications below |
Product Information
27327-1-AP targets DUSP2 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DUSP2 fusion protein Ag25380 相同性解析による交差性が予測される生物種 |
| Full Name | dual specificity phosphatase 2 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC007771 |
| Gene Symbol | DUSP2 |
| Gene ID (NCBI) | 1844 |
| RRID | AB_2880845 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DUSP2 belongs to the sub-family of nuclear specific DUSPs that includes DUSP1, 4 and 5. DUSP2 is highly expressed in immune cells, in particular, of patients with inflammatory arthritis, such as in rheumatoid arthritis (RA), and Dusp2 knockout mice present a significant reduction in inflammatory responses. It has been demonstrated that DUSP2 is a downstream target of the tumor suppressor p53 that is required for the cellular response to oxidative stress. DUSP2 is also involved in transcription factor E2F1-mediated apoptotic signaling. Like other members of the DUSP family, DUSP2 interacts with MAPKs; However, the target protein for its phosphatase activity is controversial.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP2 antibody 27327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Cell Exosomal hsa_circ_0017252 attenuates the development of gastric cancer via inhibiting macrophage M2 polarization. | ||
Basic Clin Pharmacol Toxicol Apatinib combined with Keytruda treatment induces apoptosis of gastric carcinoma cells through CES4/miR-616-5p/DUSP2 axis. | ||
Blood Coagul Fibrinolysis Effects of Momordica charantia exosomes on platelet activation, adhesion, and aggregation. | ||
Bioact Mater Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity | ||
Aging (Albany NY) Comprehensive analysis of scRNA-seq and bulk RNA-seq reveal the characteristics of disulfidptosis and a prognostic signature in BLCA |