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DUSP3 Polyclonal antibody

DUSP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28284-1-AP

Synonyms

VHR, Vaccinia H1-related phosphatase, EC:3.1.3.16, dual specificity phosphatase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP3 Polyclonal antibody (28284-1-AP)

    WB analysis using 28284-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28284-1-AP (DUSP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DUSP3 Polyclonal antibody (28284-1-AP)

    WB analysis of HeLa using 28284-1-AP

    WB result of DUSP3 antibody (28284-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DUSP3 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DUSP3 Polyclonal antibody (28284-1-AP)

    IHC staining of human stomach cancer using 28284-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28284-1-AP (DUSP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28284-1-AP targets DUSP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP3 fusion protein Ag28575 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 3
Calculated molecular weight 185 aa, 20 kDa
Observed molecular weight20 kDa
GenBank accession numberBC002682
Gene symbol DUSP3
Gene ID (NCBI) 1845
RRIDAB_2918154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DUSP3 antibody 28284-1-APDownload protocol
IHC protocol for DUSP3 antibody 28284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Immun Inflamm Dis

Dehydroandrographolide facilitates M2 macrophage polarization by downregulating DUSP3 to inhibit sepsis-associated acute kidney injury

Authors - Yanyan Shao
View Article