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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DUSP6 Polyclonal antibody

DUSP6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26071-1-AP

Synonyms

MKP3, MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP6 Polyclonal antibody (26071-1-AP)

    WB analysis using 26071-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26071-1-AP (DUSP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-12 cells using DUSP6 Polyclonal antibody (26071-1-AP)

    IP experiment of PC-12 using 26071-1-AP

    IP result of anti-DUSP6 (IP:26071-1-AP, 4ug; Detection:26071-1-AP 1:1000) with PC-12 cells lysate 1040 ug.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using DUSP6 Polyclonal antibody (26071-1-AP)

    IHC staining of mouse pancreas using 26071-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26071-1-AP (DUSP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DUSP6 Polyclonal antibody (26071-1-AP)

    IF Staining of HeLa using 26071-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using DUSP6 Polyclonal antibody (26071-1-AP)

    IF Staining of PC-12 using 26071-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHepG2 cells, PC-12 cells, mouse heart tissue
Positive IP detected inPC-12 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26071-1-AP targets DUSP6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP6 fusion protein Ag23474 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 6
Calculated molecular weight42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC003562
Gene symbol DUSP6
Gene ID (NCBI) 1848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

Protocols

Product Specific Protocols
WB protocol for DUSP6 antibody 26071-1-APDownload protocol
IHC protocol for DUSP6 antibody 26071-1-APDownload protocol
IF protocol for DUSP6 antibody 26071-1-APDownload protocol
IP protocol for DUSP6 antibody 26071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols