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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DUSP6 Recombinant antibody

DUSP6 Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240303A9

Cat no : 83355-3-RR

Synonyms

MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of PC-12 cells using DUSP6 Recombinant antibody (83355-3-RR)

    FC experiment of PC-12 using 83355-3-RR

    1x10^6 PC-12 cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using DUSP6 Recombinant antibody (83355-3-RR)

    FC experiment of HeLa using 83355-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83355-3-RR

    Affinity and Kinetic Characterization of 83355-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83355-3-RR against Human DUSP6 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive FC (Intra) detected inPC-12 cells, HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83355-3-RR is DUSP6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DUSP6 fusion protein Ag0705 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 6
Calculated molecular weight42 kDa
GenBank accession numberBC003562
Gene symbol DUSP6
Gene ID (NCBI) 1848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

Protocols

Product Specific Protocols
FC protocol for DUSP6 antibody 83355-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols