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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DUSP6 Recombinant antibody

DUSP6 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240295C8

Cat no : 83356-5-RR

Synonyms

dual specificity phosphatase 6, DUSP6, MAP kinase phosphatase 3, MKP 3, MKP3, PYST1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP6 Recombinant antibody (83356-5-RR)

    WB analysis using 83356-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83356-5-RR (DUSP6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83356-5-RR

    Affinity and Kinetic Characterization of 83356-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83356-5-RR against Human DUSP6 were performed. The affinity constant is 3.38 nM.

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Tested Applications

Positive WB detected inA431 cells, A549 cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83356-5-RR is DUSP6 Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DUSP6 fusion protein Ag23474 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 6
Calculated molecular weight42 kDa
Observed molecular weight42-45 kDa
GenBank accession numberBC003562
Gene symbol DUSP6
Gene ID (NCBI) 1848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

Protocols

Product Specific Protocols
WB protocol for DUSP6 antibody 83356-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols