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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DUSP9 Polyclonal antibody

DUSP9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26718-1-AP

Synonyms

MKP4, MKP 4, Mitogen-activated protein kinase phosphatase 4, MAP kinase phosphatase 4, EC:3.1.3.16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP9 Polyclonal antibody (26718-1-AP)

    WB analysis using 26718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DUSP9 Polyclonal antibody (26718-1-AP)

    WB analysis using 26718-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DUSP9 Polyclonal antibody (26718-1-AP)

    IHC staining of human kidney using 26718-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DUSP9 Polyclonal antibody (26718-1-AP)

    IHC staining of human kidney using 26718-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26718-1-AP targets DUSP9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP9 fusion protein Ag25167 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 9
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC060837
Gene symbol DUSP9
Gene ID (NCBI) 1852
RRIDAB_2880612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DUSP9 antibody 26718-1-APDownload protocol
IHC protocol for DUSP9 antibody 26718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma

Authors - Xiang-Xu Wang
View Article
mouseWB

Sci Rep

Inhibition of miR-194-5p avoids DUSP9 downregulation thus limiting sepsis-induced cardiomyopathy

Authors - Jie Wang
  • KD Validated
View Article